Root Border Cells and Mucilage Secretions of Soybean, Glycine Max (Merr) L.: Characterization and Role in Interactions with the Oomycete Phytophthora Parasitica.
Identifieur interne : 000072 ( Main/Exploration ); précédent : 000071; suivant : 000073Root Border Cells and Mucilage Secretions of Soybean, Glycine Max (Merr) L.: Characterization and Role in Interactions with the Oomycete Phytophthora Parasitica.
Auteurs : Marc Ropitaux [France] ; Sophie Bernard [France] ; Damien Schapman [France] ; Marie-Laure Follet-Gueye [France] ; Maïté Vicré [France] ; Isabelle Boulogne [France] ; Azeddine Driouich [France]Source :
- Cells [ 2073-4409 ] ; 2020.
Abstract
Root border cells (BCs) and their associated secretions form a protective structure termed the root extracellular trap (RET) that plays a major role in root interactions with soil borne microorganisms. In this study, we investigated the release and morphology of BCs of Glycine max using light and cryo-scanning electron microscopy (SEM). We also examined the occurrence of cell-wall glycomolecules in BCs and secreted mucilage using immunofluorescence microscopy in conjunction with anti-glycan antibodies. Our data show that root tips released three populations of BCs defined as spherical, intermediate and elongated cells. The mechanism of shedding seemed to be cell morphotype-specific. The data also show that mucilage contained pectin, cellulose, extracellular DNA, histones and two hemicellulosic polysaccharides, xyloglucan and heteromannan. The latter has never been reported previously in any plant root secretions. Both hemicellulosic polysaccharides formed a dense fibrillary network embedding BCs and holding them together within the mucilage. Finally, we investigated the effect of the RET on the interactions of root with the pathogenic oomycete Phytophthora parasitica early during infection. Our findings reveal that the RET prevented zoospores from colonizing root tips by blocking their entry into root tissues and inducing their lysis.
DOI: 10.3390/cells9102215
PubMed: 33008016
PubMed Central: PMC7650559
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France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Glycobiologie et Matrice Extracellulaire Végétale, UPRES-EA 4358, Fédération de Recherche « Normandie-Végétal »-FED 4277, Université de ROUEN Normandie, UFR des Sciences et Techniques, F-76821 Mont-Saint-Aignan</wicri:regionArea><placeName><region type="region" nuts="2">Région Normandie</region><region type="old region" nuts="2">Haute-Normandie</region><settlement type="city">Mont-Saint-Aignan</settlement></placeName></affiliation></author><author><name sortKey="Bernard, Sophie" sort="Bernard, Sophie" uniqKey="Bernard S" first="Sophie" last="Bernard">Sophie Bernard</name><affiliation wicri:level="3"><nlm:affiliation>Laboratoire de Glycobiologie et Matrice Extracellulaire Végétale, UPRES-EA 4358, Fédération de Recherche « Normandie-Végétal »-FED 4277, Université de ROUEN Normandie, UFR des Sciences et Techniques, F-76821 Mont-Saint-Aignan, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Glycobiologie et Matrice Extracellulaire Végétale, UPRES-EA 4358, Fédération de Recherche « Normandie-Végétal »-FED 4277, Université de ROUEN Normandie, UFR des Sciences et Techniques, F-76821 Mont-Saint-Aignan</wicri:regionArea><placeName><region type="region" nuts="2">Région Normandie</region><region type="old region" nuts="2">Haute-Normandie</region><settlement type="city">Mont-Saint-Aignan</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>Cell Imaging Platform (PRIMACEN-IRIB), Université de ROUEN Normandie, UFR des Sciences et Techniques, F-76821 Mont-Saint-Aignan, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Cell Imaging Platform (PRIMACEN-IRIB), Université de ROUEN Normandie, UFR des Sciences et Techniques, F-76821 Mont-Saint-Aignan</wicri:regionArea><placeName><region type="region" nuts="2">Région Normandie</region><region type="old region" nuts="2">Haute-Normandie</region><settlement type="city">Mont-Saint-Aignan</settlement></placeName></affiliation></author><author><name sortKey="Schapman, Damien" sort="Schapman, Damien" uniqKey="Schapman D" first="Damien" last="Schapman">Damien Schapman</name><affiliation wicri:level="3"><nlm:affiliation>Cell Imaging Platform (PRIMACEN-IRIB), Université de ROUEN Normandie, UFR des Sciences et Techniques, F-76821 Mont-Saint-Aignan, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Cell Imaging Platform (PRIMACEN-IRIB), Université de ROUEN Normandie, UFR des Sciences et Techniques, F-76821 Mont-Saint-Aignan</wicri:regionArea><placeName><region type="region" nuts="2">Région Normandie</region><region type="old region" nuts="2">Haute-Normandie</region><settlement type="city">Mont-Saint-Aignan</settlement></placeName></affiliation></author><author><name sortKey="Follet Gueye, Marie Laure" sort="Follet Gueye, Marie Laure" uniqKey="Follet Gueye M" first="Marie-Laure" last="Follet-Gueye">Marie-Laure Follet-Gueye</name><affiliation wicri:level="3"><nlm:affiliation>Laboratoire de Glycobiologie et Matrice Extracellulaire Végétale, UPRES-EA 4358, Fédération de Recherche « Normandie-Végétal »-FED 4277, Université de ROUEN Normandie, UFR des Sciences et Techniques, F-76821 Mont-Saint-Aignan, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Glycobiologie et Matrice Extracellulaire Végétale, UPRES-EA 4358, Fédération de Recherche « Normandie-Végétal »-FED 4277, Université de ROUEN Normandie, UFR des Sciences et Techniques, F-76821 Mont-Saint-Aignan</wicri:regionArea><placeName><region type="region" nuts="2">Région Normandie</region><region type="old region" nuts="2">Haute-Normandie</region><settlement type="city">Mont-Saint-Aignan</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>Cell Imaging Platform (PRIMACEN-IRIB), Université de ROUEN Normandie, UFR des Sciences et Techniques, F-76821 Mont-Saint-Aignan, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Cell Imaging Platform (PRIMACEN-IRIB), Université de ROUEN Normandie, UFR des Sciences et Techniques, F-76821 Mont-Saint-Aignan</wicri:regionArea><placeName><region type="region" nuts="2">Région Normandie</region><region type="old region" nuts="2">Haute-Normandie</region><settlement type="city">Mont-Saint-Aignan</settlement></placeName></affiliation></author><author><name sortKey="Vicre, Maite" sort="Vicre, Maite" uniqKey="Vicre M" first="Maïté" last="Vicré">Maïté Vicré</name><affiliation wicri:level="3"><nlm:affiliation>Laboratoire de Glycobiologie et Matrice Extracellulaire Végétale, UPRES-EA 4358, Fédération de Recherche « Normandie-Végétal »-FED 4277, Université de ROUEN Normandie, UFR des Sciences et Techniques, F-76821 Mont-Saint-Aignan, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Glycobiologie et Matrice Extracellulaire Végétale, UPRES-EA 4358, Fédération de Recherche « Normandie-Végétal »-FED 4277, Université de ROUEN Normandie, UFR des Sciences et Techniques, F-76821 Mont-Saint-Aignan</wicri:regionArea><placeName><region type="region" nuts="2">Région Normandie</region><region type="old region" nuts="2">Haute-Normandie</region><settlement type="city">Mont-Saint-Aignan</settlement></placeName></affiliation></author><author><name sortKey="Boulogne, Isabelle" sort="Boulogne, Isabelle" uniqKey="Boulogne I" first="Isabelle" last="Boulogne">Isabelle Boulogne</name><affiliation wicri:level="3"><nlm:affiliation>Laboratoire de Glycobiologie et Matrice Extracellulaire Végétale, UPRES-EA 4358, Fédération de Recherche « Normandie-Végétal »-FED 4277, Université de ROUEN Normandie, UFR des Sciences et Techniques, F-76821 Mont-Saint-Aignan, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Glycobiologie et Matrice Extracellulaire Végétale, UPRES-EA 4358, Fédération de Recherche « Normandie-Végétal »-FED 4277, Université de ROUEN Normandie, UFR des Sciences et Techniques, F-76821 Mont-Saint-Aignan</wicri:regionArea><placeName><region type="region" nuts="2">Région Normandie</region><region type="old region" nuts="2">Haute-Normandie</region><settlement type="city">Mont-Saint-Aignan</settlement></placeName></affiliation></author><author><name sortKey="Driouich, Azeddine" sort="Driouich, Azeddine" uniqKey="Driouich A" first="Azeddine" last="Driouich">Azeddine Driouich</name><affiliation wicri:level="3"><nlm:affiliation>Laboratoire de Glycobiologie et Matrice Extracellulaire Végétale, UPRES-EA 4358, Fédération de Recherche « Normandie-Végétal »-FED 4277, Université de ROUEN Normandie, UFR des Sciences et Techniques, F-76821 Mont-Saint-Aignan, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Glycobiologie et Matrice Extracellulaire Végétale, UPRES-EA 4358, Fédération de Recherche « Normandie-Végétal »-FED 4277, Université de ROUEN Normandie, UFR des Sciences et Techniques, F-76821 Mont-Saint-Aignan</wicri:regionArea><placeName><region type="region" nuts="2">Région Normandie</region><region type="old region" nuts="2">Haute-Normandie</region><settlement type="city">Mont-Saint-Aignan</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>Cell Imaging Platform (PRIMACEN-IRIB), Université de ROUEN Normandie, UFR des Sciences et Techniques, F-76821 Mont-Saint-Aignan, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Cell Imaging Platform (PRIMACEN-IRIB), Université de ROUEN Normandie, UFR des Sciences et Techniques, F-76821 Mont-Saint-Aignan</wicri:regionArea><placeName><region type="region" nuts="2">Région Normandie</region><region type="old region" nuts="2">Haute-Normandie</region><settlement type="city">Mont-Saint-Aignan</settlement></placeName></affiliation></author></analytic><series><title level="j">Cells</title><idno type="eISSN">2073-4409</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Root border cells (BCs) and their associated secretions form a protective structure termed the root extracellular trap (RET) that plays a major role in root interactions with soil borne microorganisms. In this study, we investigated the release and morphology of BCs of <i>Glycine max</i> using light and cryo-scanning electron microscopy (SEM). We also examined the occurrence of cell-wall glycomolecules in BCs and secreted mucilage using immunofluorescence microscopy in conjunction with anti-glycan antibodies. Our data show that root tips released three populations of BCs defined as spherical, intermediate and elongated cells. The mechanism of shedding seemed to be cell morphotype-specific. The data also show that mucilage contained pectin, cellulose, extracellular DNA, histones and two hemicellulosic polysaccharides, xyloglucan and heteromannan. The latter has never been reported previously in any plant root secretions. Both hemicellulosic polysaccharides formed a dense fibrillary network embedding BCs and holding them together within the mucilage. Finally, we investigated the effect of the RET on the interactions of root with the pathogenic oomycete <i>Phytophthora parasitica</i> early during infection. Our findings reveal that the RET prevented zoospores from colonizing root tips by blocking their entry into root tissues and inducing their lysis.</div></front></TEI><pubmed><MedlineCitation Status="In-Data-Review" Owner="NLM"><PMID Version="1">33008016</PMID><DateRevised><Year>2020</Year><Month>11</Month><Day>10</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">2073-4409</ISSN><JournalIssue CitedMedium="Internet"><Volume>9</Volume><Issue>10</Issue><PubDate><Year>2020</Year><Month>Sep</Month><Day>30</Day></PubDate></JournalIssue><Title>Cells</Title><ISOAbbreviation>Cells</ISOAbbreviation></Journal><ArticleTitle>Root Border Cells and Mucilage Secretions of Soybean, <i>Glycine Max</i> (Merr) L.: Characterization and Role in Interactions with the Oomycete <i>Phytophthora Parasitica</i>.</ArticleTitle><ELocationID EIdType="pii" ValidYN="Y">E2215</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.3390/cells9102215</ELocationID><Abstract><AbstractText>Root border cells (BCs) and their associated secretions form a protective structure termed the root extracellular trap (RET) that plays a major role in root interactions with soil borne microorganisms. In this study, we investigated the release and morphology of BCs of <i>Glycine max</i> using light and cryo-scanning electron microscopy (SEM). We also examined the occurrence of cell-wall glycomolecules in BCs and secreted mucilage using immunofluorescence microscopy in conjunction with anti-glycan antibodies. Our data show that root tips released three populations of BCs defined as spherical, intermediate and elongated cells. The mechanism of shedding seemed to be cell morphotype-specific. The data also show that mucilage contained pectin, cellulose, extracellular DNA, histones and two hemicellulosic polysaccharides, xyloglucan and heteromannan. The latter has never been reported previously in any plant root secretions. Both hemicellulosic polysaccharides formed a dense fibrillary network embedding BCs and holding them together within the mucilage. Finally, we investigated the effect of the RET on the interactions of root with the pathogenic oomycete <i>Phytophthora parasitica</i> early during infection. Our findings reveal that the RET prevented zoospores from colonizing root tips by blocking their entry into root tissues and inducing their lysis.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Ropitaux</LastName><ForeName>Marc</ForeName><Initials>M</Initials><Identifier Source="ORCID">0000-0002-0165-8440</Identifier><AffiliationInfo><Affiliation>Laboratoire de Glycobiologie et Matrice Extracellulaire Végétale, UPRES-EA 4358, Fédération de Recherche « Normandie-Végétal »-FED 4277, Université de ROUEN Normandie, UFR des Sciences et Techniques, F-76821 Mont-Saint-Aignan, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bernard</LastName><ForeName>Sophie</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Laboratoire de Glycobiologie et Matrice Extracellulaire Végétale, UPRES-EA 4358, Fédération de Recherche « Normandie-Végétal »-FED 4277, Université de ROUEN Normandie, UFR des Sciences et Techniques, F-76821 Mont-Saint-Aignan, France.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Cell Imaging Platform (PRIMACEN-IRIB), Université de ROUEN Normandie, UFR des Sciences et Techniques, F-76821 Mont-Saint-Aignan, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Schapman</LastName><ForeName>Damien</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>Cell Imaging Platform (PRIMACEN-IRIB), Université de ROUEN Normandie, UFR des Sciences et Techniques, F-76821 Mont-Saint-Aignan, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Follet-Gueye</LastName><ForeName>Marie-Laure</ForeName><Initials>ML</Initials><Identifier Source="ORCID">0000-0003-3199-6659</Identifier><AffiliationInfo><Affiliation>Laboratoire de Glycobiologie et Matrice Extracellulaire Végétale, UPRES-EA 4358, Fédération de Recherche « Normandie-Végétal »-FED 4277, Université de ROUEN Normandie, UFR des Sciences et Techniques, F-76821 Mont-Saint-Aignan, France.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Cell Imaging Platform (PRIMACEN-IRIB), Université de ROUEN Normandie, UFR des Sciences et Techniques, F-76821 Mont-Saint-Aignan, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Vicré</LastName><ForeName>Maïté</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Laboratoire de Glycobiologie et Matrice Extracellulaire Végétale, UPRES-EA 4358, Fédération de Recherche « Normandie-Végétal »-FED 4277, Université de ROUEN Normandie, UFR des Sciences et Techniques, F-76821 Mont-Saint-Aignan, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Boulogne</LastName><ForeName>Isabelle</ForeName><Initials>I</Initials><AffiliationInfo><Affiliation>Laboratoire de Glycobiologie et Matrice Extracellulaire Végétale, UPRES-EA 4358, Fédération de Recherche « Normandie-Végétal »-FED 4277, Université de ROUEN Normandie, UFR des Sciences et Techniques, F-76821 Mont-Saint-Aignan, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Driouich</LastName><ForeName>Azeddine</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Laboratoire de Glycobiologie et Matrice Extracellulaire Végétale, UPRES-EA 4358, Fédération de Recherche « Normandie-Végétal »-FED 4277, Université de ROUEN Normandie, UFR des Sciences et Techniques, F-76821 Mont-Saint-Aignan, France.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Cell Imaging Platform (PRIMACEN-IRIB), Université de ROUEN Normandie, UFR des Sciences et Techniques, F-76821 Mont-Saint-Aignan, France.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>09</Month><Day>30</Day></ArticleDate></Article><MedlineJournalInfo><Country>Switzerland</Country><MedlineTA>Cells</MedlineTA><NlmUniqueID>101600052</NlmUniqueID><ISSNLinking>2073-4409</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">ExDNA</Keyword><Keyword MajorTopicYN="N">bell wall</Keyword><Keyword MajorTopicYN="N">border cells</Keyword><Keyword MajorTopicYN="N">defense</Keyword><Keyword MajorTopicYN="N">heteromannan</Keyword><Keyword MajorTopicYN="N">immuno-microscopy</Keyword><Keyword MajorTopicYN="N">mucilage</Keyword><Keyword MajorTopicYN="N">polysaccharides</Keyword><Keyword MajorTopicYN="N">root</Keyword><Keyword MajorTopicYN="N">xyloglucan</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2020</Year><Month>09</Month><Day>03</Day></PubMedPubDate><PubMedPubDate 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